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​論文業績

Blood-to-testis transport of ribavirin involves carrier-mediated processes at the blood-testis

 barrier.

 Takeru Ito, Yoshiyuki Kubo, Yuma Tega, Shin-Ichi Akanuma, Ken-Ichi Hosoya; J pharm Sci, in press.

 (2024)

Development and Functional Evaluation of MDR1-Expressing Microvascular Endothelial-Like

 Cells Derived from Human iPS Cells as an In Vitro Blood-Brain Barrier Model.

​ Tomoko Yamaguchi, Daiki Sako, Toshiki Kurosawa, Misae Nishijima, Ayaka Miyano, Yoshiyuki Kubo,

 Sumio Ohtsuki, Kenji Kawabata, Yoshiharu Deguchi; J Pharm Sci, 112:3216-3223. (2023)

ヒトiPS細胞由来血液脳関門モデルの構築とトランスポーター機能評価

​ 黒澤俊樹、佐孝大樹、久保義行、出口芳春、Drug Delivery System, Vol.38 No.2 (2023)

Carrier-Mediated Process of Putrescine Elimination at the Rat Blood-Retinal Barrier.

 Yuma Tega, Yoshiyuki Kubo, Hiroaki Miura, Kairi Ri, Ayaka Tomise, Shin-Ichi Akanuma, Ken-Ichi

 Hosoya; Int J Mol Sci, 24(10):9003. (2023)

Characterization of LysoTracker Red uptake by in vitro model cells of the outer blood-retinal

 barrier: Implication of lysosomal trapping with cytoplasmic vacuolation and cytotoxicity.

 Yuma Tega, Toshinari Takeuchi, Masatoshi Nagano, Reina Makino, Yoshiyuki Kubo, Shin-Ichi

 Akanuma, Ken-Ichi Hosoya; Drug Metab Pharmacokinet, 51:100510. (2023)

Characterization of Aripiprazole Uptake Transporter in the Blood-Brain Barrier Model

 hCMEC/D3 Cells by Targeted siRNA Screening.

 Moeno Kadoguchi, Hiroshi Arakawa, Ryokichi Honda, Kazuki Hotta, Yoshiyuki Shirasaka, Yoshiharu

 Deguchi, Ikumi Tamai; Pharm Res, 39(7):1549-1559. (2022)

Professor Margareta Hammarlund‑Udenaes ‑ A Humble Scientist Shaping Modern Neuro-

 pharmacokinetics.

 Irena Loryan, David E Smith, Per Artursson, Douwe Breimer, Yoshiharu Deguchi, Lennart

 Paalzow, Yuichi Sugiyama, Tetsuya Terasaki, Elizabeth C M de Lange; Pharm Res,

 39(7):1283-1289. (2022)

Proteomics-Based Transporter Identification by the PICK Method: Involvement of TM7SF3

 and LHFPL6 in Proton-Coupled Organic Cation Antiport at the Blood–Brain Barrier.

 Toshiki Kurosawa, Yuma Tega, Yasuo Uchida, Kei Higuchi, Hidetsugu Tabata, Takaaki Sumiyoshi,

 Yoshiyuki Kubo, Tetsuya Terasaki, Yoshiharu Deguchi; Pharmaceutics, 14(8), 1683. (2022)

Functional characteristics of 3'-azido-3'-deoxythymidine transport at the blood-testis barrier

 Takeru Ito, Yoshiyuki Kubo, Shin-Ichi Akanuma, Ken-Ichi Hosoya; Int J Pharm., 25;625:122044.

 (2022)

Naltrexone Transport by a Proton-Coupled Organic Cation Antiporter in hCMEC/D3 Cells, an

 In Vitro Human Blood-Brain Barrier Model.

 Atsushi Kitamura, Kei Higuchi, Toshiki Kurosawa, Takashi Okura, Yoshiyuki Kubo, Yoshiharu Deguchi;

 Biol Pharm Bull., 45(10):1585-1589. (2022)

Construction and Functional Evaluation of a Three-Dimensional Blood-Brain Barrier Model

 Equipped With Human Induced Pluripotent Stem Cell-Derived Brain Microvascular

 Endothelial Cells.

 Toshiki Kurosawa, Daiki Sako, Yuma Tega, Yasuyuki Debori, Yumi Tomihara, Kazunobu

 Aoyama, Yoshiyuki Kubo, Nobuyuki Amano, Yoshiharu Deguchi; Pharm Res., 39(7):1535-

 1547. (2022)

​・Involvement of TauT/SLC6A6 in Taurine Transport at the Blood-Testis Barrier.

 Yoshiyuki Kubo, Sakiko Ishizuka, Takeru Ito, Daisuke Yoneyama, Shin-Ichi Akanuma, Ken-Ichi

 Hosoya; Metabolites., Jan 12;12(1):66. (2022)

Transport Characteristics of 6-Mercaptopurine in Brain Microvascular Endothelial Cells

 Derived from Human Induced Pluripotent Stem Cells.

 Toshiki Kurosawa, Yuma Tega, Daiki Sako, Tatsuki Mochizuki, Tomoko Yamaguchi, Kenji Kawabata,

 Katsuhisa Inoue, Naoki Ito, Hiroyuki Kusuhara, Yoshiharu Deguchi; J Pharm Sci​., Oct; 110(10)

 :3484-3490. (2021)

Functional investigation of solute carrier family 35, member F2 (SLC35F2) in three cellular

 models of the primate blood-brain barrier.

 Tatsuki Mochizuki, Tadahaya Mizuno, Toshiki Kurosawa, Tomoko Yamaguchi, Kei Higuchi, Yuma Tega,

 Yoshitane Nozaki, Kenji Kawabata, Yoshiharu Deguchi, Hiroyuki Kusuhara; Drug Mtab Dispos., Jan

 49(1):3-11. (2020)

Structural Requirements for Uptake of Diphenhydramine Analogs into hCMEC/D3 Cells Via

 the Proton-Coupled Organic Cation Antiporter.

 Yuma Tega, Hidetsugu Tabata, Toshiki Kurosawa, Atsushi Kitamura, Fumio Itagaki, Tetsuta

 Oshitari, Yoshiharu Deguchi; J Pharm Sci., Jan 110(1):397-403. (2020)

Fibronectin is essential for formation of fenestrae in endothelial cells of the fenestrated

 capillary.

 Takashi Nakakura, Takeshi Suzuki, Hideyuki Tanaka, Kenjiro Arisawa, Toshio Miyashita, Yoko Nekooki-

 Machida, Toshiki Kurosawa, Yuma Tega, Yoshiharu Deguchi, Haruo Hagiwara; Cell Tissue Res., Feb

 383(2):823-833. (2020)

Discovery of a Blood-Brain-Barrier Permeable Histone Deacetylase Inhibitior.

 Takaaki Sumiyoshi, Seiya Hiranaka, Yoshiharu Deguchi, Akihiro Ito, Minoru Yoshida; MEDCHEM NEWS

 Vol. 30 No. 1 (2020)

Expression and functional characterization of SLC transporters in brain microvascular

 endothelial cells derived from human induced pluripotent stem cells.

 Yuma Tega, Toshiki Kurosawa, Kei Higuchi, Tomoko Yamaguchi, Takashi Nakakura, Tatsuki Mochizuki,

 Hiroyuki Kusuhara, Kenji Kawabata, Yoshiharu Deguchi; Abstracts from the 13th International

 Conference on Cerebral Vascular Biology (CVB 2019), Fluids and Barriers of the CNS, 16 (2019)​

Expression and Functional Characterization of Drug Transporters in Brain Microvascular

 Endothelial Cells Derived from Human Induced Pluripotent Stem Cells.

 Kurosawa T, Tega Y, Higuchi K, Yamaguchi T, Nakakura T, Mochizuki T, Kusuhara H, Kawabata K, and

 Deguchi Y; Mol. Pharmaceut.,15(12), 5546-5555 (2018)

Transport of Pregabalin Via L-Type Amino Acid Transporter 1 (SLC7A5) in Human Brain

 Capillary Endothelial Cell Line.

 Takahashi Y, Nishimura T, Higuchi K, Noguchi S, Tega Y, Kurosawa T, Deguchi Y, and Tomi M; Pharm

 Res. 35(12), 246 (2018)

Impact of Nicotine Transport across the Blood-Brain Barrier: Carrier-Mediated Transport of

 Nicotine and Interaction with Central Nervous System Drugs.

 Tega Y, Yamazaki Y, Akanuma SI, Kubo Y, and Hosoya KI.; Biol Pharm Bull., 41(9),1330-1336 (2018)

Design, synthesis, and blood-brain barrier transport study of pyrilamine derivatives as

 histone deacetylase inhibitors.

 Hiranaka S, Tega Y, Higuchi K, Kurosawa T, Deguchi, Y, Arata M, Ito A, Yoshida M, Nagaoka Y, and

 Sumiyoshi T.;ACS Med. Chem. Lett., 9(9), 884-888 (2018)

Recent advances in research on biological membranes that regulate the central nervous

 system.

 Nakanishi T, Tamai I, and eguchi Y.; Biol. Pharm. Bull. 41(9), 1322-1323 (2018)

Prostaglandin Transporter OATP2A1/SLCO2A1 Is Essential for Body Temperature Regulation

 during Fever.

 Nakamura Y, Nakanishi T, Shimada H, Shimizu J, Aotani R, Maruyama S, Higuchi K, Okura T,

 Deguchi Y, and Tamai I.; J. Neurosci. 38(24), 5584-5595 (2018)

Involvement of proton-coupled organic cation antiporter in varenicline transport at blood-

 brain barrier of rats and in human brain capillary endothelial cells.

 Kurosawa T, Higuchi K, Okura T, Kobayashi K, Kusuhara H, and Deguchi Y.; J. Pharm. Sci., 106(9),

 2576-2582 (2017)

Functional expression of nicotine influx transporter in A549 human alveolar epithelial cells.

 Tega Y, Yuzurihara C, Kubo Y, Akanuma SI, Ehrhardt C, Hosoya KI. Drug Metab. Pharmacokinet.,

 31(1), 99-101(2016)

Histamine elimination from the cerebrospinal fluid across the blood-cerebrospinal fluid

 barrier: Involvement of plasma membrane monoamine transporter (PMAT/SLC29A4).

 Usui T, Nakazawa A, Okura T, Deguchi, Y, Akanuma SI, Kubo Y, Hosoya K; J Neurochem. 139,

 408-418. (2016)

Cocktail-dosing microdialysis study to simultaneously assess delivery of multiple organic-

 cationic drugs to the brain.

 Kitamura A, Higuchi K, Okura T, Deguchi Y; J. Pharm. Sci. 105, 935-940.(2016) 

Involvement of the H+/organic cation antiporter in nicotine transport in rat liver.

 Tega Y, Akanuma S, Kubo Y, Hosoya K. Drug Metab. Dispos., 43(1), 89-92 (2015)

Carrier-Mediated Transport of Nicotine Across the Inner Blood-Retinal Barrier: Involvement

 of a Novel Organic Cation Transporter Driven by an Outward H(+) Gradient.

 Tega Y, Kubo Y, Yuzurihara C, Akanuma S, Hosoya K. J. Pharm. Sci., 104(9), 3069-3075 (2015)

The blood-brain barrier transport mechanism controlling analgesic effects of opioid drugs in

 CNS.

 Okura T, Higuchi K, Deguchi Y; Yakugaku Zasshi. 135, 697-702 (2015).

Memantine transport by a proton-coupled organic cation antiporter in hCMEC/D3 cells, an in

 vitro human blood-brain barrier model.

 Higuchi K, Kitamura A, Okura T, Deguchi Y; Drug Metab Pharmacokinet., 30 (2), 182-187 (2015)

Endothelin-1 receptors in rat tissues: characterization by bosentan, ambrisentan and CI-

 1020.

 Yoshinari Yokoyama, Ayaka Osano, Hideki Hayashi, Kunihiko Itoh, Takashi Okura, Yoshiharu

 Deguchi, Yoshihiko Ito, Shizuo Yamada; Biol. Pharm. Bull., 37 (3), 461-465 (2014)

Fetal Growth Retardation and Lack of Hypotaurine in Ezrin Knockout Mice.

 Nishimura T, Higuchi K, Sai Y, Sugita Y, Yoshida Y, Tomi M, Wada M, Wakayama T, Tamura A, Tsukita

 S, Soga T, Nakashima E; PLoS One. 9 (8), e105423 (2014)

Transport characteristics of tramadol in the blood-brain barrier.

 Atsushi Kitamura, Kei Higuchi, Takashi Okura, and Yoshiharu Deguchi; J. Pharm. Sci., 103 (10),

 3335-3341 (2014)

Functional expression of organic cation/carnitine transporter 2 (OCTN2/SLC22A5) in human

 brain capillary endothelial cell line hCMEC/D3, a human blood-brain barrier model.

 Okura T, Kato S, Deguchi Y; Drug Metab Pharmacokinet., 29 (1), 69-74 (2014)

System A amino acid transporter SNAT2 shows subtype-specific affinity for betaine and

 hyperosmotic inducibility in placental trophoblasts.

 Nishimura T, Yagi R, Usuda M, Oda K, Yamazaki M, Suda S, Takahashi Y, Okazaki F, Sai Y, Higuchi K,

 Maruyama T, Tomi M, Nakashima E; Biochim Biophys Acta., 1838( 5),1306-1312 (2014)

Proton-Coupled Organic Cation Antiporter-Mediated Uptake of Apomorphine Enantiomers in

 Human Brain Capillary Endothelial Cell Line hCMEC/D3.

 Okura T, Higuchi K, Kitamura A, Deguchi Y; Biol Pharm Bull., 37 (2), 286?291 (2014)

Bladder endothelin-1 receptor binding of bosentan and ambrisentan.

 Osano A, Yokoyama Y, Hayashi H, Itoh K, Okura T, Deguchi Y, Ito Y, Yamada S; J Pharmacol Sci.,

 124(1), 86-91 (2014)

Muscarinic receptor binding of the novel radioligand, [(3)h]imidafenacin in the human  

 bladder and parotid gland.

 Yoshida A, Kuraoka S, Ito Y, Okura T, Deguchi Y, Otsuka A, Ozono S, Takeda M, Masuyama K, Araki I,

 Yamada S; J. Pharmcol. Sci., 124 (1), 40-46 (2014)

Ependymal cells of the mouse brain express urate transporter 1 (URAT1).

 Tomioka NH, Nakamura M, Doshi M, Deguchi Y, Ichida K, Morisaki T, Hosoyamada M; Fluids Barriers

 CNS., 10 (1), 31. doi:10.1186/2045-8118-10-31. (2013)

Evaluation of rat in vivo fetal-to-maternal transfer clearances of various xenobiotics by

 umbilical perfusion.

 Nishimura T, Takanohashi T, Tomi M, Horikoshi M, Higuchi K, Sai Y, Nakashima E; J Pharm Sci.,102

 (9), 3356-3363 (2013)

Estrogen receptor α induction by mitoxantrone increases Abcg2 expression in placental

 trophoblast cells.

 Oda K, Nishimura T, Higuchi K, Ishido N, Ochi K, Iizasa H, Sai Y, Tomi M, Nakashima E; J Pharm

 Sci.,102(9), 3364-3372 (2013)

Functional expression of a proton-coupled organic cation (H+/OC) antiporter in human brain

 capillary endothelial cell line hCMEC/D3, a human blood-brain barrier model.

 Keita Shimomura, Takashi Okura, Sayaka Kato, Pierre-Oliver Couraud, Yoshiharu Deguchi; Fluid

 Barrier CNS, 10:8,(2013).

Characterization of the Carrier-mediated Transport of Ketoprofen, a Nonsteroidal Anti-

 Inflammatory Drug, in Rabbit Corneal Epithelium Cells.

 Shinobu Fujii, Hideki Hayashi, Kunihiko Itoh, Shizuo Yamada, Yoshiharu Deguchi and Kouichi Kawazu;

 J. Pharm. Pharmacol., 65, 171-180 (2013)

Blood-to-brain influx transport of nicotine at the rat blood-brain barrier: involvement of a

 pyrilamine-sensitive organic cation transport process.

 Tega Y, Akanuma S, Kubo Y, Terasaki T, Hosoya K. Neurochem. Int., 62(2), 173-81 (2012)

Measurement of glomerular filtration rate by rapid intravenous injection of a newly

 developed insulin fraction.

 Yoshitaka Maeda, Yuya Araki, Tomomi Uno, Akiho Yoshida Keisuke Nishigaki, Naoto Inaba, Hiroaki

 Hayashi, and Yoshiharu Deguchi; J. Rural Med, 6(1), 9-15 (2011)

The blood-brain barrier as a focus of drug metabolism and pharmacokinetics.

 Yoshiharu Deguchi; Drug Metab. Pharmacokinet. 26 (3), 1 (2011)

Diphenhydramine active uptake at the blood-brain barrier and its interaction with oxycodone   

 in vitro and in vivo.

 Muhammad Waqas Sadiq, Annika Borgs, Takashi Okura, Keita Shimomura, Sayaka Kato, Yoshiharu

 Deguchi, Britt Jansson1, Sven Bjorkman, Tetsuya Terasaki, Margareta Hammarlund-Udenaes; J.

 Pharm. Sci., 100 (9), 3912 - 3923 (2011)

Functional characterization of rat plasma membrane monoamine transporter in the blood-

 brain and blood-cerebrospinal fluid barriers.

 Takashi Okura, Sayaka Kato, Yusuke Takano, Takenori Sato, Atsushi Yamashita, Riyo Morimoto,

 Sumio Ohtsuki, Tetsuya Terasaki, Yoshiharu Deguchi; J. Pharm. Sci. 100 (9), 3924 - 3938 (2011)

Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats.

 Higuchi K., Iizasa H, Sai Y, Horieya S, Lee K. E, Wada M, Deguchi M, Nishimura T, Wakayama T,

 Tamura A, Tsukita S, Kose N, Kang Y. S, and Nakashima E; Biol. Pharm. Bull., 33(8), 1400-1406

 (2011).

Drug-drug interaction between oxycodone and adjuvant analgesics in blood-brain barrier

 transport and antinociceptive effect.

 Yusuke Nakazawa, Takashi Okura, Keita Shimomura, Tetsuya Terasaki, Yoshiharu Deguchi; J. Pharm.

 Sci., 99, 467-474 (2010).

Effects of dietary ingredients on function and expression of P-glycoprotein in human

 intestinal epithelial cells.

 Takashi Okura, Michiko Ibe, Keizo Umegaki, Kazumasa Shinozuka, Shizuo Yamada; Biol. Pharm. Bull.

 33, 255-259 (2010)

A possible mechanism for the decrease in serum thyroxine level by a TCDD-like PCB

 congener, 3,3',4,4',5- pentachlorobiphenyl in mice.

 Yoshihisa Kato, Koichi Haraguchi, Makio Kubota, Yoshiki Seto, Takashi Okura, Shin-ichi Ikushiro,

 Nobuyuki Koga, Shizuo Yamada, Masakuni Degawa; Drug Metab. Dispos. 38, 150-156 (2010)

Genetic deficiency of carnitine/organic cation transporter2 (slc22a5) is associated with

 altered tissue distribution of its substrate pyrilamine in mice.

 Sayaka Kato, Yukio Kato, Tadakatsu Nakamura, Tomoko Sugiura, Yoshiyuki Kubo, Yoshiharu Deguchi,

 and Akira Tsuji; Bio. Drug Dispos., 39, 495-507 (2009).

Effects of repeated morphine treatment on the antinociceptive effects, intestinal absorption,

 and efflux from intestinal epithelial cells of morphine.

 Takashi Okura, Tadahiro Ozawa, Michiko Ibe, Yuko Taki, Midori Kimura, Yoshiyuki Kagawa, Yoshihisa

 Kato, Shizuo Yamada; Biol. Pharm. Bull. 32, 1571-1575 (2009)

Effects of quinidine on antinociception and pharmacokinetics of morphine in rats.

 Takashi Okura, Yuki Morita, Yoshihiko Ito, Yoshiyuki Kagawa, Shizuo Yamada; J. Pharm. Pharmacol.

 61, 593-597. (2009).

Involvement of Pyrilamine Transporter, a Putative Organic Cation Transporter, in Blood-Brain

 Barrier Transport of Oxycodone.

 Takashi. Okura, Asami. Hattori, Yusuke Takano, Takenori Sato, Margareta Hummerlund-Udenaes,

 Tetsuya Terasaki and Yoshiharu Deguchi; Drug Metab. Dispos., 36(10), 2005 - 2013 (2008).

Enhancement by grapefruit juice of morphine antinociception.

 Takashi Okura, Tadahiro Ozawa, Yoshihiko Ito, Midori Kimura, Yoshiyuki Kagawa, Shizuo Yamada;

 Biol. Pharm. Bull. 31, 2338-2341 (2008)

Comparative Analysis of Partition Coefficients in Linear and Nonlinear eliminating organs

 without measurering tissue concentrations.

 H. Ito, N. Kose, Y. Deguchi, L. Z. Benet, E. Nakashima, J. Kyoritsu Univ. Pharm., 3, 9-18 (2007).

Blood-brain barrier transport of pramipexole, a dopamine D2 agonist.

 Takashi Okura, Rie Ito, Naoki Ishiguro, Ikumi Tamai and Yoshiharu Deguchi; Life Sci., 80: 1564-1571

 (2007).

Comparative measurement of spinal CSF microdialysate concentrations and concomitant

 antinociception of morphine and morphine-6b-glucuronide in rats.

 T Okura, N Komiyama, Y Morita, M Kimura, Y Deguchi and S Yamada; Life Sci., 80:1319-1326

 (2007).

Prediction of α1-adrenoceptor occupancy in the human prostate from plasma concentrations

 of silodosin, tamsulosin and terazosin to treat urinary obstruction in benign prostatic

 hyperplasia.

 Shizuo Yamada, Yasuhiro Kato, Takashi Ohkura, Yoshiyuki Kagawa and Kazuki Kawabe; Biol. Pharm.

 Bull. 30, 1237-1241 (2007)

Different effects of morphine and morphine-6β-glucuronide on formalin-evoked spinal

 glutamate release in conscious and freely-moving rats.

 Takashi Okura, Noriyuki Komiyama, Yuki Morita, Midori Kimura, Shizuo Yamada; Neurosci. Lett. 415,

 169-173 (2007)

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